Beckman Coulter Cytomics FC 500 Руководство пользователя - Страница 8
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Quality Control check on the FC500
Every morning (Monday - Friday), Facility staff run 2 QC bead sets to ensure that the
lasers & PMTs are functioning normally. The QC beads also check to make sure the
lasers have "warmed up" enough and are functioning at the appropriate power level.
Very rarely do we see results that are abnormal - and usually when we do the problem is
either
a) a bubble in the fluidics (solved by priming several times)
-or-
b) some miscommunication between the computer & the cytometer (solved by shutting
off the cytometer and restarting; or by shutting down the cytometer & computer and
restarting. When this happens, after shutting down I use "FC On (Warmup)" to turn on
the FC500, not the "CXP Cytometer" icon. Then, once the FC500 is on and the "laser-
ready" arrow shows up on the front panel, I then open the CXP software).
If you wish to run the QC beads to ensure accuracy of your results, please follow these
instructions:
1. After turning on the FC500, wait for the "laser-ready" arrow to show up on the front
panel.
2. After logging in to CXP software, open the "1A_beads 9434031" Acquisition Protocol
under the FLOW user.
3. In the refrigerator (top shelf, left-hand side) is a flow sample tube with tape around it
that says "FC500 & XL FlowCheck 9434031". Vortex, then run this sample under the
beads 9434031 protocol.
4. A bunch of events should show up in the rectangular gate in the FS vs SS plot. Also, a
peak should show up in each of the linear gates in the histrograms. If not, abort and then
try priming twice. Try to run the tube again. If still no luck after more priming, shut
down & restart.
5. Assuming the events & peaks show up, after starting the sample, RESTART after
about 5 seconds. Then let the sample run til it automatically stops at 5000 events.
6. The HP X-CVs for each linear gate should be no more than 1.90. The normal range
for each FL channel is listed on the inside cover of the FC500 Log Book vol. 2 binder,
sitting on top of the bookshelf on the other side of hte wall from the telephone.
7. If the HP X-CVs are outside of the acceptable range, prime and then run the tube
again. Usually this fixes it. If the CVs remain high, something is wrong with the optics,
laser(s) or fluidics, and you should contact a Facility staff member (phone numbers &
emails will be posted by the cytometer).