Active Motif PIXUL Руководство пользователя - Страница 10

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Warning: A 96-Well PIXUL Plate (cat. no. 53139) should be mounted and secured prior to attempt-

ing to circulate the PIXUL Coupling Fluid. Load your samples into the 96-Well PIXUL Plate in the

same orientation as will be indicated on the instrument touchscreen. Any wells lacking sample

in the columns being sonicated MUST be filled with liquid (water or buffer). Seal the plate well.

Coupling Fluid should be circulated and allowed to cool to approximately 15°C prior to initiating

sample processing.

1. Ensure that the PIXUL Coupling Fluid level is about an inch (2.5 cm) below the reservoir top. Do

not overfill.

2. Switch on the power switch on the back side of the PIXUL instrument.

3. Press the main power button on the front side of the PIXUL instrument. The touchscreen will

start initializing.

4. Load a sample-containing 96-Well PIXUL Plate (cat. no. 53139) into the PIXUL instrument. Lift

the external lid, place the plate with well A1 in the upper left corner, secure down the pressure

distribution lid on top of the plate with plate-securing rods, and close the external lid.

5. On the touchscreen, press Circulate to initiate PIXUL Coupling Fluid cooling. You cannot start

the sonication run with out circulation active. You can monitor Coupling Fluid temperature in the

upper right hand corner of the touchscreen.

6. On the touchscreen, select the plate columns for which you would like to set sonication param-

eters. Columns selected together will be outlined in the same color.

7. On the left side of the touchscreen, you can use the left and right arrows to select from saved

presets of sonication parameters. You can also use the add and delete buttons to add or remove a

row of process settings.

Adjust the following parameters (See Appendix, Section A for further details):

Pulse: The number of 2 MHz cycles per pulse

PRF: Pulse Repitition Frequency; the kHz frequency of pulse repititions

Process Time: Time a given column is actively driven. Process timers for all columns run si-

multaneously, so the total run time for the entire plate is equal to the time of the column(s)