Olympus CX31 Руководство по эксплуатации - Страница 16

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4-5 Condenser

Fig. 21

Fig. 22

Fig. 23

1. With the 10X objective engaged and the specimen brought into focus,

turn the field iris diaphragm ring @ counterclockwise to stop down the

diaphragm to near its minimum size.

2. Turn the condenser height adjustment knob ² to bring the field iris

diaphragm image into focus.

3. Rotate the two condenser centering knobs ³ to adjust so that the field

iris diaphragm image is centered in the eyepiece field of view. (Figs. 21

& 22)

4. To check centration, open the field iris diaphragm until its image

touches the perimeter of the field of view. If the image is not precisely

inscribed in the field of view, center again. (Fig. 22)

5. When used for actual observation, open the field iris diaphragm until its

image is slightly larger than the field of view.

· The aperture iris diaphragm determines the numerical aperture of the

illumination system. Matching the numerical aperture @ of the

illumination system with that of the objective provides better image

resolution and contrast, and also increases the depth of focus.

Adjustment method

Align the aperture iris diaphragm knob ² with the NA value @ on the

scale. The scale value should correspond to the NA number engraved

on the objective.

Since the aperture iris diaphragm knob has a certain width, align the

center line engraved on the knob with the scale indication.

When using a 100X objective, turn the aperture iris diaphragm knob ²

all the way to 0.9 on the scale. (Fig. 23)

· Since the contrast of microscope specimens is ordinarily low, setting

the condenser aperture iris diaphragm to between 70% and 80% of

the N.A. of the objective in use is usually recommended.

#If the aperture iris diaphragm is set too small, image ghost may be

observed.

(Figs. 21 & 22)

(Fig. 23)