Olympus CX33 Руководство по эксплуатации - Страница 26

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Aperture diaphragm

The aperture diaphragm is a diaphragm to adjust the numerical aperture of the condenser.

Adjusting the numerical aperture of the condenser appropriately with respect to the numerical aperture of each objective

allows you to observe the specimen with the best contrast and resolution.

In general, since the contrast of the specimen to be observed

with microscope is low, it is appropriate to adjust the

numerical aperture of the condenser to approximately 70 to

80% of the numerical aperture of the objective.

The rough indication is that the aperture diaphragm image

viewed when looking into the tube without eyepieces looks as

the picture shown on the right.

Objective

Plan objective

Numerical aperture of objective and numerical aperture of condenser suitable for observation

Magnification

10X

20X

40X

100XO

30-20%

Numerical aperture of

objective

0.10

0.25

0.40

0.65

1.25

70-80%

Aperture diaphragm image

Pupil of objective

(bright circular ring)

Numerical aperture of condenser suitable for

observation of specimen for microscope

(when setting to 70% of objective)

0.10 x 0.7 = 0.07

0.25 x 0.7 = 0.175

0.40 x 0.7 = 0.28

0.65 x 0.7 = 0.455

1.25 x 0.7 = 0.875