Olympus Fluoview FV1000 Manuallines
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Olympus FV1000 confocal microscope
Please
read and follow our
•
don´t use without introduction by
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Only switch on parts you need for your purposes
•
(e.g. if you don't need the Hg lamp for epifluorescence like for overnight on confocal
microscopes)
report problems to
•
Laserlines:
Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
Red Diode Laser: 635 nm (Cy5, Alexa 633/647)
Switching on the system:
Switch on the PC (button next to floppy drive).
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Switch on the microscope components with the power manager (Mic-
•
control, PSU1+2, HBO lamp if neccessary)
Switch on the lasers:
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A) Laser combiner (FV10-MCPSU)
B) 559 nm laser if needed
1) Main switch
2) Wait until 'TEMP' is not blinking anymore
3) Turn key to 'on' position
.
guidelines
us
without delay
ALMF
staff