Olympus Fluoview-1000 Руководство пользователя - Страница 5

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V.M. Bloedel Hearing Research Center, Core for Communication Research

Center on Human Development and Disability, Digital Microscopy Center

A. Start-up:

All items are lettered in the order in which they are to be turned on.

1. Enter your name and session into the Olympus FV-1000 web calendar;

2. Turn on items labeled with letters:

(A) Turn on mercury lamp, if needed - power supply is on shelf over monitor;

(B) Turn on system controllers – press black switch on power supply on right side of air table, next

to the computer.

the lasers) (405 nm, 561 nm, 633 nm);

(D) Fire up the DPSS laser (561 nm) – press the green button;

(E) Turn on Argon ion laser – turn the key to "ON" (458 nm, 488 nm, 514 nm);

(F) Turn on computer, black tower to right of confocal, press round button to right of floppy;

a. Windows user name is "Administrator"

b. password is "fluoview"

3. Double-click on the Fluoview icon, left side of computer monitor desktop, "FV-10ASW";

4. Choose your name from the users list and enter your password;

NOTE: Fluoview user accounts open with the last settings used by the previous user.

B. Shut down:

1. Transfer files from your account (See File Transfer, Section );

2. Exit from Fluoview;

3. Ignore the warning about light coming through the microscope.

4. Turn off power strip (B);

5. Turn off power strip (C);

6. Turn off argon ion laser (E), key only;

7. Delete images from the hard drive;

8. Turn off the computer;

Remove immersion oil from objectives

10. Edit time in the web calendar, if you went longer than reserved.

Keys versus Switches.

Use the key to turn the argon laser on and off. Do not use the power switch – that will prevent the fan to

run during cooling down and potentially damage the laser.

For components controlled by power strips (B, C, D), do not fiddle with the keys or switches on the

actual components. The exception is that the 561 nm laser (D) needs its green button pushed to turn it

on, but it is turned off by the power strip (C), along with the other 2 lasers.

If the building power fails, backup batteries will provide about 10 minutes to turn off all components.

May 11, 2011

Olympus Fluoview-1000 User's Guide

Quick Start Guide

, clean the stage and platform.