Nikon Elcipse TE2000 Руководство по эксплуатации - Страница 7
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TROUBLESHOOTING
If you get imaging problems, check the following:
Is there a shadow in the image with transmitted light illumination?
If you are using bright-field microscopy the condenser must be adjusted
correctly to Kohler illumination [otherwise you might see the dark shadow of
the phase rings in the centre of the image and get poor image quality]. Ask a
core member how to set up Kohler illumination on the microscope.
Alternatively an incorrectly set filter slider or other component may be
obscuring the light path.
Is the mercury bulb or transmission bulb turned on?
Check the orange and green lights on the power boxes and the microscope
white button/brightness control. Check light can be seen at the specimen.
Can you see a coloured light passing through the bright field [phase-
contrast] sample?
↔
Move the filter wheel to the bright field double arrow
position.
(
)
Is the fluorescence filter block in place? Are you using the correct filter
block – do you need it ?
Rotate the filter wheel and see if any light of the right colour appears.
Have you checked the dial below the eyepiece?
It should be set to O.
Is the objective correctly positioned?
The objective wheel should gently click into position.
Is the port/optical path in the correct position?
Port 1 is for observation down the microscope. Port 5 is for the Hamamatsu
B&W camera only. Port 4 is split 50/50 between the microscope and the
Nikon colour camera.
Is the light too faint or too bright?
Remove/Add the neutral density filters. Ensure the black T-A polariser slider
isn't pushed in [left side, under the objectives] unless you need it.
FOR ANY QUERIES OR DOUBTS, JUST ASK
A MEMBER OF THE CORE STAFF
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