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Nikon Labophot Руководство по эксплуатации

v .

PHOTOMICROGRAPHY

(The

Biological

Microscope

LABOPHOT

designed mainly for observation.)

1. Combination of CF Objectives and

Projection lens

The combined use of the CF objectives and CF

PL Projection lens is essential.

For

the same total

magnification,

select a

combination

of the highest possible objective

power

and

lowest

possible projection

lens

power to achieve the utmost image definition

and contrast.

2. Checking the Illumination

Uneveness in the illumination

will

show up

more conspicuously in photomicrography

than

in observation. Consequently, before taking a

photograph, recheck the correct adjustment of

the condenser.

3. Selection of Voltage and Filter

1) When using a daylight type color fil m

Set the brightness control dial to 5.5 and use

the NCB 10 fi Iter

*-'

Adjustment

of the image brightness should be

made by means of the NO filters.

2) When using a monochrome

film

Remove the NCB 10 fi Iter. Contrast fi Iters such

as X-1 green are usable.

*-'

The NCB 10 filter is most suitable for a

standard film. Depending upon the make

of the film different color renditions may

result. It is recommended that in addition

to the NCB 10 filter a color compensation

filter

(CC filter),

available from the film

manufacturer, be used.

4. Shutter Speed

Desirable shutter speeds for least vibration are

1/4~

1/15sec.

Adjustment

of the image brightness for color

photomicrography

should be made by means of

the NO filters.

Some specimens require, on

account of their insufficient

brightness, longer

exposure times, and consequently

poor color

reproducibility

owing to the "Reciprocity

Law

Failure"

of film may result. So, when taking

picture of such specimens, it is recommended

to use the Nikon Biological microscope OPTI-

PHOTo

5. Manipulation of Field and Aperture

Diaphragms

In photomicrography,

the adjustment

of the

field diaphragm is important for the purpose of

limiting extraneous light which causes flare in

the microscope

image. Stop down the dia-

phragm so as to get an illuminated area slightly

larger than that of the picture field. By adjust-

ing the aperture diaphragm, a change of depth

of focus, contrast and resolution

of image is

attainable. Select a size suited to the purpose.

Generally speaking, the aperture diaphragm, is

properly stopped down to 70 ~ 80% of the

aperture of the objective being used.

6. With Regard to Condensers

For

photomicrography,

is generally

rec-

ommended

to use the Swing-out Achromat

condenser. When using 2X objective, however,

preferab Iy remove the condenser.

7. Focusing

Focusing is to be accomplished by means of the

ocular finder on the photomicrographic

attach-

ment, or binocular observation tube with mask

eyepiece on the trinocular eyepiece tube.

Table 3.

Focusing

Type of

Focusing with

eyepiece

10x or 4 x or

tube

higher objective

lower objective

Use

Focusing

"F" tube

Ocular

magnifier

finder

fi nder

Use

"T"

observation

Use

tube

observation

Focusing

magnifier

"UW" tube

tube

Ocular

finder