Nikon Labophot Руководство по эксплуатации - Страница 24
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Failures
Causes
)
Actions
• Out of focus
) • For preventing
external
vibration.
use vibration-
(Especially
with high
proof
table or rigid desk.
power objective
and
• Select a place free from vibrations.
such as caused
e)
by traffic.
passers-by or motors etc.
bration-.
Using NO fi Iters or others. elongate exposure
time
(for color film. to 1/4 -1/15
sec.)
•
Lower the voltage. and elongate exposure
time (for
black·and·white
film).
Note. however.
for color film. that lowering
of
color temperature
and change of spectral character-
istics will be unavoidable.
ness----+.
Use a standard coverglass of 0.17mm
in thickness.
of coverglass
(No.1
%)
hen
•
Use objective
With coverglass thickness
compensa-
N.A. and
tion ring.
jective)
• Using dry objectives---+.
Use no·coverglass
type objective.
for smear preparations
•
If other objectives
are to be used. place a coverglass
on the specimen.
• Grease. dust or dirt----+.
Clean the front
of objective
thoroughly.
top surface
on optical
surfaces
of projection
lens. specimen.
photographic
lens.
condenser
lens. field lens. etc.
• Condenser
not
).
Centering
(Refer to P. 10)
centered
(This shows up
more conspicuously
in photography
than
in observation)
nt
• Aperture
diaphragm---+.
Generally.
good resu Its wi II be achieved with
opened too large
aperture
stopped
down to 70 - 80% of N.A. of the
objective
being used. (Refer to P. 10)
of
).
In metallurgical.
interference.
polarizing
or phase
contrast
microscopy.
use of a green fi Iter or mono-
chromatic
interference
filter
(e.g. peak wavelength
= 546nm.
half-value
range = 30 nm) will
increase
contrast.
• When contrast
is to be increased for a part stained
with a particular
color. use a filter
whose color is
complementary
to the stain color (for black-and-
white film).
e of~.
Stop down field diaphragm
to a diameter
slightly
field diaphragm
larger than the diagonal
of picture
frame.
(Refer to P. 16)
24