Nikon N-STORM Руководство по эксплуатации - Страница 29
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(5)
Upon the completion of the test analysis, the
confirmation dialog is displayed. Click [OK].
Make sure that the bright points are identified
correctly.
If the intended result is not achieved
If bright points of noise are detected or if bright points of
signals are not detected, reconfigure the parameters for
identifying bright points in
and then perform the test analysis again.
To analyze only some periods
Using the period slider, the analysis target periods can
be specified by right-clicking each of the start and end
points of the periods to be analyzed and by then
selecting [Set as Start Period] and [Set as End Period].
(6)
Click
[Start STORM Analysis] again.
(7)
Click [Start] to start analysis of the entire dataset.
Analysis of bright points is performed for the entire
dataset. Upon the completion of the analysis, the
confirmation dialog is displayed.
(8)
Click [OK].
As soon as the analysis is complete, information on
the positions of the molecules is saved in the
following two formats.
• Molecule list in binary format
File name: (ND2-file-name)_list.bin
• Drift correction information
File name: (ND2-file-name) +_drift.txt
Both the binary file and the text file are saved to the
same location as the ND2 file of the dataset.
Overwriting confirmation dialog
If the result of a past analysis of the analyzed ND file exists in the same folder, a dialog like that shown below is
displayed.
To save the result of a previous analysis, select [Yes]. The file name for the results of the previous analysis is
changed to [(ND2-file-name)_list-(the-current-date-and-time-yyyy-mm-dd-hh-mm-ss).bin]. The results of the latest
analysis are saved as [(ND2-file-name)_list.bin].
If the result of the previous analysis is unnecessary, select [No]. The result of the latest analysis is saved as
[(ND2-file-name)_list.bin], overwriting the result of the previous analysis.
To move or copy files
When moving or copying files for STORM, handle the ND2 file and the binary (bin) and text (txt) files of the analysis
result as a group.
Chapter 2
STORM Microscopy Operation
[Identification Settings]
25
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