AmScope B270 Manuel - Sayfa 20

Mikroskop AmScope B270 için çevrimiçi göz atın veya pdf Manuel indirin. AmScope B270 20 sayfaları.

B 2 7 0 User Gui de

2.5 Immersion Oil

Certain high-magnification objective lenses with numerical-apertures over 1.0 will be marked with the word "oil." This

signifies that the lens is designed to work with specialized oil as an immersion medium. The immersion oil is used be-

tween lenses and specimens to eliminate air from the optical path in order to maintain high resolution. This oil is only

intended to be used with compatible lenses, and should not be used with non-oil lenses. The most-common lens to use

oil is a 100X objective lens with a numerical aperture of 1.25 or higher. A small drop of oil is placed on the specimen,

then the lens would be rotated into place. The specimen must be appropriately prepared to allow the use of oil, such as

by using a cover slip. When the stage is raised to achieve focus, the objective lens will make contact with the oil.

Immersion oil should also be used with oil-compatible condensers. Condensers with numerical apertures over 1.0 must

use an immersion medium to achieve the high numerical apertures. A drop of immersion oil would be placed on the con-

denser's top lens, then the condenser would be raised so the oil makes contact with the underside of the specimen. This

technique should only be used with specimens which have been properly prepared, such as being mounted on a glass

slide.

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