BD FACSAria Başlatma, Qc ve Kapatma Prosedürü - Sayfa 6
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4. Optics QC
1
In Diva, go to QC Folder and open the 3-Peak Beads Experiment
1
1
Create a new tube and name it with the date: yyyymmdd
2
1
Run the CS&T beads in the 3 peak experiment
3
To prepare CS&T beads, add one drop of BD CS&T beads, Cat#655050, into 350uL of 1xPBS.
(Refrigerate after use.)
1
Verify that all channels have 3 peaks and brightest peak is falling within target.
4
For each laser, the primary fluorescent parameter should have an %rCV below.
Open 3-Peak Beads
Experiment.
Verify in the
statistics box that
the bright peak on
the primary
channel for each
laser is below 6.0
1
Record 5,000 events.
5
5. Stream Sort QC and Accudrop
Verify quality of charging the stream.
1
Turn on voltage
and press Test Sort