BD LSR II Kullanıcı Kılavuzu
Ölçüm Cihazları BD LSR II için çevrimiçi göz atın veya pdf Kullanıcı Kılavuzu indirin. BD LSR II 8 sayfaları. Flow cytometry analyzer
Ayrıca BD LSR II için: Kullanıcı Kılavuzu (20 sayfalar)
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PPE must be worn at all times in our BSL-2 environment
*Lab coat, gloves, and closed toed shoes are mandatory; respirator and goggles are available*
An Instrument Configuration is located on the website in the
Do I need to run beads when I collect data using 'Diva Digital'?
For each data collection, you need to first collect data on 197 calibration beads. This is to ensure that the
instrument is running properly.
What samples do I need to bring?
• All users ALWAYS need to bring single stained compensation controls
• If you have a 7 color panel you should bring: 8 compensation controls
o One tube stained with each individual antibody
o One unstained tube
o No PI or viability dye in the single color control
Don't have enough cells for compensation?
User can purchase compensation beads from many different suppliers
• eBioscience UltraComp Beads 01-2222-42 (for panels including UV and Violet dyes)
• eBioscience OneComp Beads 01-1111-42 (for panels that do NOT include any UV or Violet dyes)
• BD CompBeads 552845 'Anti-Rat/Hamster' (for most anti-mouse antibodies)
• BD CompBeads 552843 'Anti-Mouse' (for most anti-human antibodies)
• ThermoFisher Amine Reactive Compensation Beads A10628 (for LIVE/DEAD Fixable stains)
Starting Up the System
*Allow 30 minutes for the lasers to warm up*
1. Turn on the Cytometer.
a. The power switch is located on the lower right corner on the Cytometer.
b. The fluid control button lights are GREEN and the STANDBY button light is amber.
2. SSFF computers are left on at all times.
a. If computer is turned off, log into Windows with User Name: FACS User
i. There is no password
LSR II Users Guide
Stanford Shared FACS Facility
Instrument Overview area
*The configuration lists the lasers and filters for each machine*
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