A-M Systems 3000 Kullanım Kılavuzu - Sayfa 10

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MODE (STIM-GATE-REC)

Ω

TEST

3. Turn on power to the Model 3000 and allow it to warm up for 5 minutes.

4. Non Headstage option: connect measuring electrode to the black wire and a

reference electrode to the white wire of the probe cable.

Headstage option: connect measuring electrode to the +

input of the head stage and a reference electrode to the -

input of the head stage (see diagram to the right). Note: the

reference electrode can be tied to the gnd input on the head

stage. Clamp the Head stage in a micromanipulator.

5. Dip the measuring and reference electrode into a beaker of

physiological saline solution (or the solution in which the

tissue will be bathed). The solution should have the same

temperature and ionic strength as that in which

measurements will be made. Note: immerse the measuring electrode to

approximately the same depth as will be used during the measurement.

6. Connect an oscilloscope to the

2ms/division. Zero the scope. Flip the input switch to

7. Observe the offset potential between the two electrodes on the oscilloscope. Set

the

DC OFFSET (+ OFF -)

knob to zero the amplifier output. Note: make sure your oscilloscope is in DC

mode.

8. Turn on the Ω

electrode.

9. Adjust the oscilloscope for a good display of the square-wave. The Oscilloscope

will display a square wave at 0.1 V peak to peak for every 1 MΩ of electrode

resistance.

10. Increase the

CAPACITY COMPENSATION

Avoid overcompensation, which will cause ringing, excessive noise, and high

frequency oscillation.

11. Turn the

CAPACITY COMPENSATION

present position and the off position. This will minimize the chance of

overcompensation during recording.

12. Turn off the Ω

13. Apply the electrodes to the experimental preparations.

14. Apply the

HIGH PASS, LOW PASS,

15. Increase the gain switch until the output voltage is in the range necessary for

saving data. Note: The

A-M Systems

OUTPUT

switch to the appropriate polarity and adjust the

button to inject a 100 Hz square-wave current through the

TEST

switch to stop the test signal.

TEST

and

Switch should be set so that the signal at the

GAIN

131 Business Park Loop, P.O. Box 850 Carlsborg, WA 98324

Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525

E-mail: [email protected] * Website:

REC

OFF

BNC, with the horizontal sweep rate set to

DIFF.

to "square-up" the corners of the waveform.

knob to approximately half way between its

if necessary.

NOTCH FILTERS

http://www.a-msystems.com

DC OFFSET

OUTPUT