Olympus BX-RFAA Talimatlar Kılavuzu - Sayfa 15

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Fig. 2

Centering the Aperture Iris Diaphragm

1. Engage the shutter in the light path by pressing the RSHT button on the

hand switch.

2. Engage the B or IB mirror unit in the light path by pressing the MU+ or

MU– button on the hand switch.

(If these mirror units are not available, engage another fluorescence mir-

ror unit in the light path.)

3. Engage the 10X objective in the light path by pressing the objective

button, then place the U-CST centering target on the stage.

4. Release the shutter by pressing the RSHT button.

5. Move the white surface with crosslines of the U-CST until the crosslines

are overlaid on the center of field.

6. Engage the empty place (the objective cap should be removed) in the

light path.

7. Pull out the aperture iris diaphragm knob @ to minimize the aperture iris

diameter.

8. Pull out the field iris diaphragm knob ² to minimize the field iris dia-

phragm. Now the aperture iris image should be visible on the U-CST.

9. Fit the Allen screwdrivers in the two aperture iris centering screws ³ and

adjust so that the aperture iris image coincides with the crosslines.

Effects of Aperture Iris Diaphragm

The aperture iris diaphragm helps adjust the brightness of the observed

image and improve the contrast.

To perform normal fluorescence observation, enlarge the aperture iris by

pushing in the aperture iris diaphragm knob @.

}If specimen colors tend to fade due to too high excitation light, first use

ND filters to reduce the brightness, and decrease the aperture iris dia-

phragm if the ND filters are not enough.

Do not decrease the aperture iris diaphragm too much. Do not use it as

a substitute to the shutter.

(Fig. 2)