Olympus BX41 Talimatlar Kılavuzu - Sayfa 19

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Aperture iris

diaphragm image

Objective pupil

Compatibility of Objectives and Condensers

Objective

Magnification

1.25X

10-60X

100X**

* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture iris

diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the

surroundings of the field of view may be dark but observation is still possible.

** When using the U-AC2 Abbe condenser, the field iris diaphragm image cannot be viewed with a 100X objective.

}To obtain better illumination in photomicrography using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.

70-80%

30-20%

Fig. 31

N.A. scale

Fig. 32

Abbe

U-AC2

Applicable to FN 22

Applicable

(FN 26.5)

Aperture Iris Diaphragm

The aperture iris diaphragm determines the numerical aperture of the

illumination system. Matching the numerical aperture of the illumination

system with that of the objective provides better image resolution and

contrast, and also increases the depth of focus.

Since the contrast of microscope specimens is ordinarily low, setting the

condenser aperture iris diaphragm to between 70% and 80% of the N.A.

of the objective in use is usually recommended. If necessary, adjust the

ratio by removing the eyepiece and looking into the eyepiece sleeve

while adjusting the aperture iris diaphragm ring @ until the image shown

in Fig. 31 is seen.

}Using the numerical aperture scale:

Set the condenser numerical aperture scale to about 80% of the NA

value ² of the respective objective. (Fig. 32)

Example: With the UPlanFI40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6.

Condenser

Achromat/aplanat

U-AAC

Applicable to FN 22

Applicable by moving

top lens out of the light

path.* (FN 26.5)

Applicable

Engage top lens in

(FN 26.5)

light path (FN 26.5)

(Figs. 31 & 32)

Swing-out

Ultralow magnification

U-SC3

U-ULC-2

Applicable

(FN 26.5)