Olympus U-PCD2 Talimatlar Kılavuzu - Sayfa 7

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# The other phase annuluses should be centered in the same manner.

In case Ph1 is indicated on both 10X and 20X objectives, for observation purposes, once the phase

annulus is aligned for 10X, it's unnecessary to realign the phase annulus for 20X.

However, for the purpose of photomicrography, it is recommended the phase annulus be aligned for

each objective even though the same Ph number may be indicated on the objective lens.

7. Remove the U-CT30, and insert the eyepiece back into the eyepiece sleeve.

Now you can start your phase contrast observation with any phase contrast objective and corresponding

light annulus as desired.

Darkfield Observation

1. Rotate the turret until "DF" can be seen in the central window.

2. Engage the 10X or higher objective.

# Objectives with NA 0.7 or lower NA and objectives with iris diaphragm can be used.

3. Place the specimen on the stage and move the stage up and down to bring the specimen into focus.

4. Move the condenser up and down until uniform darkfield illumination is attained.

5. Open the field iris diaphragm to the extent that even brightness is attained.

# Keep eyes away from the eyepieces while changing the objective during darkfield observation, or

changing from darkfield to another observation mode.

U-PCD2