Nikon eclipse e100 Talimatlar Kılavuzu - Sayfa 35

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Optical Characteristics

Combinations of 10x (Field No. 18) Eyepiece with

Objectives

Objective

Total

Magnification Magnification Aperture

(1) Total Magnification

The total magnification of a microscope is the individual magnifying power of the objective

multiplied by that of the eyepiece.

(2) Numerical aperture (N.A.)

The numerical aperture is an important factor in determining the efficiency of the condenser

and objective. It is represented by the formula:

N.A. = n sin

where n is the refractive index of the medium (air, immersion oil, etc.) between the

objectivelens and the specimen or condenser, and

light enters or leaves the lens from or to a focused object point on the optical axis.

The larger the numerical aperture the brighter the image and the higher the resolution.

(3) Resolving Power

The ability of an optical system to discriminate between two discrete objects separated by a

minute distance. The more minute the distance, the higher the resolving power of the optical

system. In relation to the numerical aperture, the resolving power is represented by the

following formula:

Resolving power =

where

is the used wavelength of light. (The resolving power in the above table is indicated

for

= 0.55 m.)

(4) Working Distance (W.D.)

The clearance between the front of the objective and the upper surface of the coverglass,

when a specimen image is sharply focused. Generally, the higher the magnifying power of the

objective, the shorter the working distance.

Numerical

Real

Viewfield Focus

is half of the maximum angle at which

Depth of

Resolving

Power

Working

Distance