Nikon Eclipse Ti TIFT 1454 Manuel - Sayfa 4

Mikroskop Nikon Eclipse Ti TIFT 1454 için çevrimiçi göz atın veya pdf Manuel indirin. Nikon Eclipse Ti TIFT 1454 9 sayfaları. Microscope

Finding focus

• Put the objective in the lowest position using button A

o Lowest position is

• Choose the objective with the right magnification

• Put a little drop emersion oil on the lens

o Only if it's an oil emersion objective

• Place the metal ring in the middle of the heating chamber

o Secure with the metal clips

• Centre you cells above the objective with the joystick

o Button on top of joystick will change speed, S (slow) or F

(fast)

• Turn button C until the oil is in contact with the coverslip

• In MetaMorphs Taskbar choose "transmission to eyes" to

illuminate the cells with the halogen lamp

• With button D you can use the fine adjustment control knob

course, fine or extra fine

• Look through the eyepiece and bring cells in focus.

o The display of the microscope shows the height of the objective

• Finding appropriate fluorescence cells can be done with the mercury lamp

o Choose the illumination settings for your fluorophore in the Taskbar in Metamorph

o Increase or decrease brightness by changing the ND filters

o ND 4, 8, 10 will block intensity, the can be combined, use minimal intensity to reduce

bleaching of your fluorophore

• When your sample is in focus you can focus the beam of the laser if necessary, for detailed

explanation ask a designated OIC member

• To change coverslips, bring objective down with lowest button A. Bring objective back to

previous height with upper button A

Perfect Focus System (PFS)

• If focus is found a LED (E) will turn orange and/or you will hear a beep

o Focus can be kept using the Perfect Focus system

o Press the green blinking button E

• The focus of the PFS is adjustable with a module as shown opposite

• The turntable navigate the lens up and down as shown and with the blue button toggles

between fine (pressed) and coarse

• Focusing with perfect focus should be viewed through the camera

The buttons FGH give the direction of the output signal:

F: eyepiece

Martijn de Gruiter

500 um

G: camera left QuantEM:512SC

Manual: TIRF Microscope

H: camera port right

Erasmus OIC

Ee-1454