Nikon Live Cell 2 Manuel - Sayfa 8

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Image Acquisition

As with the settings for viewing the sample through the eye-port, optical

configurations are employed to make changes to the selected filters and the

light path. Further to this, the optical configurations also save the detector

settings which may vary between configurations on multichannel samples.

When starting Elements software the correct camera driver needs to be

selected (note that it is possible to select both). After logging in it is possible to

select the required driver from the dropdown menu: Nikon DS-Qi2 for

brightfield/fluorescence imaging, Nikon DS-U3 for colour imaging (e.g.

histological stainings). (Fig. 17)

Acquiring a colour image – DS-U3 camera.

1. Select the colour camera BF optical configuration from the OC

Panel (Fig. 16 )

2. Focus on the sample and adjust the detector settings so that you

can see the surface.

3. Set the transmitted light to 100%.

4. Move to a blank region of the sample (Fig.19) and select Auto

White in the DS-Fi2 setting window (Fig. 18). This will ensure that

your images have the correct white balance (Fig.20).

5. Move back to your sample to image it with the correct colour

representation. If your Kohler illumination is set correctly

acquisition time for the detector should be <100ms and the gain

should be <10x. Ensure that high quality capture is selected for

your images. (It can be useful to use the Auto Exposure option to

set the exposure time.)

6. Click the capture button or run relevant ND acquisition.

Fig. 19

Nikon Imaging Centre @ King's College London

Nikon

Imaging Centre

Fig. 20

King's College London

Fig. 17

Fig. 18