Etaluma LS460 Başlangıç Kılavuzu - Sayfa 6

Laboratuvar Ekipmanları Etaluma LS460 için çevrimiçi göz atın veya pdf Başlangıç Kılavuzu indirin. Etaluma LS460 14 sayfaları. Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)

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computer and the other square USB-B end into the square port labeled TO COMPUTER on the back of

the LS560 (see photo above). We highly recommend use of the same USB port on your computer every

time you connect to the LS560. It is also best to connect the LS560 directly to the computer USB port

rather than through a USB hub.

If Lumaview has not been run previously, it is important that the LS560 and computer be connected

using the USB cable before launching Lumaview. This is because Windows needs to load the USB

drivers before Lumaview can be run. After the first time, the program can be launched before the

LS560 and computer are connected. In the latter case, only the Outer Main Window will open (see

Section II.B. below). Connecting the LS560 and computer with the USB cable will result in opening of

the Live Image Window as well.

L. Live Cell Imaging With an LS Microscope In an Incubator

1. Prior to imaging in an incubator, place the LS560 inside for 6-12 hr to allow equilibration to the

incubator temperature.

2. Place the sample on the LS560, focus, and close the incubator door. After 60 minutes, check the focus.

If satisfactory, imaging can be initiated.

3. When live cell imaging at magnifications of 40x and above, the focus can sink several microns as the

mechanism relaxes. If the image has drifted out of focus, estimate the amount of focus knob rotation

required to bring the image back into focus and overcompensate the same amount past the point of

focus. Check the focus again after 60 minutes. The image should be in focus and imaging can be

initiated. Repeat process as needed.

II. Software: Getting Started With Lumaview

The following sections give instructions for getting started with Lumaview on the LS560 and are intended as

a quickstart guide only. Not all options are given and not all features may be explained in any particular

section. The complete User Guide for Lumaview is contained in the Help Section after Lumaview is opened.

Click on Help in the Title Bar to open the pulldown menu and then click Contents, or simply Click F1 to open

the Help Section. Becoming familiar with the complete Help Section is a must for using the LS560

optimally and taking advantage of the many features of this Microscope and accompanying software.

CAUTION: When any LED is turned on, do NOT look directly at the light. Be sure to turn off all LEDs at the

end of a session so any sample remaining on the deck is not photo-bleached.

A. Starting Conditions

1. Lumaview has been downloaded to the computer.

2. The LS560 is connected to the computer via the UBS cable.

3. Sample to be imaged is present on the LS560 stage (and fluorescence shroud is over sample if imaging

involves fluorescence; not applicable to the LS460).

B. Launching Lumaview

2017.11.20

LS560/460 Startup Guide