BD FACSAria Процедура запуску, контролю якості та вимкнення - Сторінка 8
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Check Sort Precision.
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Precision should be at "Fine Tune" in 2 Tube mode.
"Fine Tune" selected
Load & Run Accudrop beads.
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To prepare Accudrop beads, put one drop of BD Accudrop beads, Cat#345249, into 700uL of 1xPBS.
(Refrigerate after use.) Acquire the beads and run at a flow rate of ~2,000 evts/sec.
Carry out Accudrop sort.
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Click "Sort" followed by "Cancel" when the dialog box comes up. Turn on the voltage manually. Confirm
that there is no fanning and that the Accudrop laser diode is aligned.
Turn on Optical Filter.
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Click "Optical Filter" and then adjust the stream deflection so the sort stream falls within the filter.
Set Drop-charge Delay
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Adjust Drop-charge Delay value up and down in the Side Stream window until the left stream is >98%.
Note: Percentages in center stream and left stream should add to 100%
Increase optimal drop-charge delay 1 whole number. Verify that left stream is ≤2%.
Decrease optimal drop-charge delay 1 whole number. Verify that left stream is ≤ 2%.
TIP: Hold down control and press arrow up or down to change values by one whole integer.
Prepare for sort.
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Adjust side streams for deflection into appropriate collection vessels and begin your experiment.
For plate sorting
See FACSAria™ Drop-Charge Delay SOP for more detailed instructions
BD FACSAria™ Plate Sorting SOP.
See
Drop-charge Delay