Beckman Coulter Cytomics FC 500 Посібник користувача - Сторінка 11
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Start-up Procedure:
1. Verify that the Sheath Fluid container is full. Fill with Sheath Fluid to the blue
line.
2. Verify that the waste container is empty. If it contains waste, carefully dump into
sink and add bleach to the first line (bleach is under sink)
3. Ensure that the printer is filled with paper. Fill with paper from the shelf if not.
4. Turn on printer with the switch on the back of the printer.
5. If the computer is not on, turn it on. If needed, log in to the computer (see yellow
note on screen).
6. Turn on power to the monitor screens.
7. Turn on the cytometer by double-clicking the "FC ON" icon on the computer
Desktop
8. The lasers will require about 15 minutes to warm up before the cytometer is ready
to be used. The lasers are ready when a green arrow-head appears on the front
display of the FC500.
9. Use one of the workstation computers to log-in to RIMS: Double click and open
the "RIMS Login" on the desktop and log-in
a. Log in to RIMS using your PSU user ID and password
b. Click on "New Login" under "Lab Time" and log in with your desired
Project
c. Back on the home screen, you can now click on your reservation time
under "Equipment – My Reservations." Or, click on "Use Equipment"
under "Equipment." Log in with your desired Project
d. It is okay to now log off of the RIMS website (and stay logged IN to the
equipment) by clicking the door icon in the upper right corner. Or, it is
also okay to remain logged in to the website.
10. Double click and open the "CXP Cytometer" icon on the Desktop
the User and enter the Password (Password: password), then click "Next"
11. Click on a Protocol, if desired; then click "Finish"
12. Ensure that the cytometer is not in Idle mode; if it is, click on the Idle mode icon
13. Click the Prime icon
before the instrument is ready to run, as sometimes an air bubble will get trapped
in the flow cell during the shutdown/startup procedures. If an air bubble is
trapped, the cells in the sample tube will flow through the cytometer but will not
be detected. Thus, pay attention as the first sample tube is run to ensure that the
cells are being detected.
. It may be necessary to click this icon multiple times
, select