Major Science MJ-105A Інструкція з експлуатації - Сторінка 12
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4.2 Start the operation
Gel Preparation
1.
Add the appropriate amount of agarose powder and 1x TAE or 1x TBE
solution into a glass flask or beaker based on desired ratio.
2.
Dissolve the agarose powder by heating the agarose either on a magnetic
hot plate with a stirring bar or in a microwave oven. If using the microwave
method, the microwave should be set at around 400W or medium setting
with the flask swirling once every minute. The solution should be heated until
all crystals are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere with sample
migration if not completely dissolved.
Note: The gel is suggested to be cooled to between 50°C and 60°C degrees before
pouring.
3.
Install the Gel Tray in the Gel Maker Stand.
4.
Select the suitable Analytical Comb for the application.
5.
Pour approx. 25 to 30 ml of agarose solution for a large tray; pour 12 to 15
ml for a small tray. The tray contains the agarose level for reference.
Note: Pour the agarose carefully so as not to generate bubbles. Any bubble that
surfaces could be smoothed to the edge of the gel and dispersed using a
pipette tip.
6.
Place the comb(s) into the grooves.
7.
As for the MJ-105 series, both Gel Maker Stands can be connected together
for user convenience.
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