Olympus BX53 Посібник користувача - Сторінка 5
Переглянути онлайн або завантажити pdf Посібник користувача для Мікроскоп Olympus BX53. Olympus BX53 9 сторінок. Polarizing microscope
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LM
MW Microscop
py Laboratory
3. Le
et's take mult
ticolor fluores
and T
TxRed fluorop
phores.
4. Cl
lick the Proce
ess Manager t
multicolor whe
m
eel
button
th
he list. Repea
at twice to sel
ne
ext to the cam
mera icon; it w
bu
utton. If Auto
omatic expos
ex
xposure time
. Once the ex
co
onditions for
DAPI.
5. Cl
lick the FITC s
section on the
op
ptimal exposu
ure time and
ch
hannel. Once
all the settin
fo
or each chann
nel automatic
as
s .vsi file in th
he Libraries>P
Sa
ave the origin
nal image files
6. Im
mages can als
o be saved as
to
o change the i
image into 8‐
as
s one image c
containing mu
sa
ave the image
e as multiple i
7. Sc
cale bar can b
be viewed wit
di
isplayed with
View/Info St
im
mage. Save th
he annotated
y
scence image
e acquisition w
f
tab
and ch
eck the Auto
n. Click the
lect FITC (gre
en) and TxRe
will expand to
o show curren
ure (with Spo
ot 30%) is use
xposure cond
ditions are set
e list and use
the Automat
click
icon
n to read the
gs are obtain
ed, click the
ally. The resu
ulting multich
Pictures folde
er as soon as t
s as .vsi forma
at without an
s .tiff files. Se
elect Image>M
‐bit image. Us
se File>Save a
ultiple channe
els. Or use Fil
images for ea
ach channel.
th View/Scale
e bar comma
tamp comma
and. These an
image as JPE
EG(JFIF)(*.jpg
O l y
with an exam
ple stained w
g
matic Proces
sses
. Click
button
n and select D
d (red) chann
nels. Click the
nt exposure s
setting. Click
ed, let the pro
ogram find th
t, click
ic
on to set the
tic exposure.
Wait until it f
setting. Rep
eat once mor
butt
ton. It will ta
hannel image
e, by default, w
the acquisitio
on has been c
ny manipulatio
on or process
Mode>8 bit/C
Channel men
as... menu co
mmand to sa
le>Export to>
>tiff series... c
nd and the m
magnification
nnotations ca
an't be saved
).
m p u s B X
X 5 3 U s e r
with DAPI, FIT
TC,
k the
DAPI (blue) in
e "plus" sign
the Live
e appropriate
e
se exposure
finds an
re for TxRED
ke three shot
ts
will be saved
completed.
sing.
nu command
ave the image
e
command to
info can be
f
with your tiff
f
G u i d e
| 5
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