Olympus FLUOVIEW FV300 Огляд - Сторінка 7

Переглянути онлайн або завантажити pdf Огляд для Мікроскоп Olympus FLUOVIEW FV300. Olympus FLUOVIEW FV300 16 сторінок. Confocal laser scanning biological microscopes

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FRET

Hardware and software support to optimize the environment for FRET.

Ratio changes when cameleon is manifested on the HeLa cell and stimulated by histamine then inhibited by

cyproheptadine.

Cameleon genes provided by Dr. Miyawaki Atsushi in Brain Research Institute.

Equipment: FV300 and HeCd laser

Time period : 4 seconds.

CFP Fluorescence wavelength 485nm

CFP/YFP FRET

Calcium ion concentration in a live HeLa cell using a cameleon (split type) indicator. Energy transfer between

CFP and YFP is proportional to bound calcium. The time series shows the increase of calcium ion density

caused by stimulation of histamine and the effect of blocking by proheputajin.

YFP Fluorescence wavelength 530nm

Measurement

The 440nm diode laser can be added

for CFP/YFP FRET imaging.

A 440nm diode laser is optionally available

for CFP/YFP imaging. The 440nm laser line

ideally excites CFP, with minimal

disturbance to YFP, and is therefore

suitable for CFP/YFP FRET experiments.

The high performance LSM objectives,

PLAPO40XWLSM and PLAPO60XWLSM,

are precisely corrected in this wavelength

range, and ensure the highest measuring

reliability.

*For simultaneous observation of CFP and YFP, 440nm

and 515nm laser lines are required.

Ratio imaging to analyze

2-wavelength images

Using time course software, the ratio image

can be continuously displayed in pseudo-

color. At the same time, the intensity of

each channel can be monitored graphically.

The analysis process is presented as an

intuitive flow chart. (optional time course

software: TIEMPO)

Input/output of external trigger signal

The optional time course software gives

control over the input/output trigger signal

by GUI. It is suitable for combined

experiments such as those involving patch

clamping.