Nikon N-STORM Посібник з експлуатації - Сторінка 37

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Nikon N-STORM Посібник з експлуатації
15
Calculate the parameters for calibration and then save
them as settings.
(1)
Click
[Identification Settings] to display the
dialog box and then click [Z axis Calibration].
(2)
Click [Auto Calibrate].
The calibration results (coefficients of the calibration curve
function) obtained through the procedure above are
automatically saved as internal settings in the N-STORM
software, and subsequently are automatically applied to
3D-STORM analysis.
Check the results of calibration on the graph
Clicking [Graph] in the [Z Calibration] dialog box displays
a graph showing the results of calibration. Display the
graph of [Wx/Wy vs. Z] if necessary, and check that Wx
and Wy intersect nearly at 0 on the horizontal axis and
that there is no large gap between the plot data
(distributed points) and the fitting data (lines).
Apply the results of calibration to an existing
dataset
To apply the new calibration result to an existing dataset,
apply the following procedure.
(1) Click [Save] in the [Z Calibration] dialog box to save
the calibration result as a file.
(2) Open the existing dataset.
(3) Display the [Z Calibration] dialog again and then click
[Load] to read the calibration result file to be applied.
Click [OK] twice to close the dialog box.
(4) Click
[Start STORM Analysis] to perform
analysis again.
Timing of calibration
When the objective has been switched or the
composition of the STORM buffer has been changed,
perform calibration again.
Chapter 2
STORM Microscopy Operation
15-(2)
33
15-(1)