Nikon N-STORM Посібник з експлуатації - Сторінка 5

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Chapter

Terminology Used in This Document

N-STORM

System that allows STochastic Optical Reconstruction Microscopy (STORM) with Nikon's inverted research microscope

Ti-E.

During STORM, some fluorescent probe molecules are randomly stimulated (activated) with relatively weak light to become

activated, after which their images are acquired through an EM-CCD camera (imaging). The series of images that is acquired

through frequent repetition of this process is analyzed and synthesized by software, and formed into a super-resolution

image.

Conventional image

Image of 256 x 256 pixels acquired using an EM-CCD camera. In STORM analysis, a set of sequentially acquired

conventional images is used as material.

STORM image (2D-STORM/3D-STORM image)

Super-resolution image generated as a result of analyzing a dataset of conventional images. It includes information such as

the position, size, and intensity of each individual fluorescent probe molecule.

A STORM image that only has information on the positions in the X- and Y-axis directions is referred to as "2D-STORM

image," while one that also has information on the positions in the Z-axis direction is referred to as "3D-STORM image."

Dye pair

Composite dye used for STORM observation, such as the following:

Examples: Alexa405-Alexa647

Cy2-Alexa647

Cy3-Alexa647

Probe

Fluorescent molecule (dye pair or monomolecular dye) used for STORM observation.

Dataset

Set of consecutive frames (conventional images) acquired through an EM-CCD camera. A dataset is saved in ND2 file format

for NIS-Elements.

Frame

Individual conventional image that is acquired through an EM-CCD camera as material to be used to generate a STORM

image.

Activation frame

Frame (image) that is acquired when relatively weak laser light for activation is emitted. This activation causes some

fluorescent probe molecules to become activated. Activation frames are not used for STORM analysis. Note that if images

are to be acquired in continuous mode, there is no difference between the activation and imaging frames because activation

and imaging are performed simultaneously.