Nikon Optiphot Короткий довідник - Сторінка 2

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Details on Getting Started With The Microscope

See the Nikon Optiphot manual for additional information about the microscope.

1. Epi-fluorescence.

1.1. When using fluorescence, turn on the mercury lamp before anything else;

1.2. The xenon arc lamp power supply sits on the shelf over the microscope;

1.3. Press the rocker switch labeled "POWER" towards "I", the green light will come on;

1.4. Press the button labeled "IGNITION" for about 3-4 seconds, never more than 10 seconds;

1.5. The amber "LAMP READY" flashes, then is steady in 1-2 minutes, after lamp stabilizes;

1.6. If the amber indicator doesn't come on in 3 minutes, turn off the power, wait 5 minutes then

proceed from step 1.2;

1.7. If the bulb fails to ignite, it may be too hot from prior use, the bulb may be faulty or may

need to be reseated. Contact facility staff.

2. Transmitted Illumination with halogen lamp (Figure 1).

2.1. Power switch turned on by the black switch, left front corner of the microscope base;

2.1.1. This lamp is used for brightfield, phase contrast, and darkfield microscopy

2.2. Lamp intensity is controlled by 3 means:

2.2.1. Lamp voltage controlled by slider at front of the microscope base

2.2.2. Neutral density filters mounted below the condenser (Figure 1, Table 2)

2.2.2.1.

2.2.3. Photo Button – sets lamp to 9V, which is much too bright for the digital camera

3. Beam Splitter (Figure 3, Table 1)

3.1. 3 options for dividing the light between the eyepieces and camera port

Position

Middle

Fully out

Table 1. Beamsplitter positions.

July 29, 2016

Digital Microsopy Center, University of Washington

Avoids need to change lamp voltage, which alters white balance

100% to eyepieces

14% to eyepieces/86% to camera

100% to camera

Start-up

Image path