Clontech PT3139-1 Посібник користувача - Сторінка 7
Переглянути онлайн або завантажити pdf Посібник користувача для Лабораторне обладнання Clontech PT3139-1. Clontech PT3139-1 20 сторінок. Advantage-hf pcr kit
Друкувати сторінкуII. List of Components
Advantage-HF PCR Kit (#K1909-1[100 rxns], 1909-y [10 rxns])
Store all components at –20°C. Enough reagents are supplied for 100 PCR
reactions of 50 µl each.
10 rxns 100 rxns
µ
µ
• 10
l 100
µ
•
60
l 600
µ
•
60
l 600
µ
•
60
l 600
µ
• 400
l
4 ml Purified Water (Millipore-purified)
µ
•
10
l 100
µ
•
10
l
40
TEL:415-424-8222 or 800-662-CLON
FAX:415-424-1064 or 800-424-1350
l
50X Advantage-HF Polymerase Mix
Includes KlenTaq-1 DNA polymerase and TaqStart Antibody
(1.1 µg/µl) in the following storage buffer.
Concentration
in 50X
50 %
40 mM
50 mM
25 mM
1 mM
5.0 mM
0.25 %
TM
Deep Vent
is a minor component of the Advantage-HF
R
Polymerase Mix.
µ
l 10X HF PCR reaction buffer
µ
l 10X cDNA PCR reaction buffer
µ
l 10X HF dNTP mix
µ
l Control DNA template
λ DNA (0.2 ng/µl)
µ
l Control primer mix (10 µM each)
The sequences are:
5' primer 5'–TTGGTTGATCGTGGTGCAGAGAACGTTG–3'
3' primer 5'–GAGAAGGTCACGAATGAACCAGGCGATAA–3'
Technical Support
Component
Glycerol
Tris-HCl (pH 7.5)
KCl
(NH
)
SO
4
2
4
EDTA
β-mercaptoethanol
Thesit
CLONTECH Laboratories, Inc.
1X
Concentration
1.0 %
0.8 mM
1.0 mM
0.5 mM
20 µM
0.1 mM
0.005 %
Protocol # PT3139-1
Version # PR76834
page
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