Astrea Evolve AA20CRK Ersatz-Handbuch - Seite 12

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Reassembly of 200mm and 250 column.

Reassembly of the column is the reverse of disassembly.

Tube Assembly

1. The orientation of the tube is shown by the fitted scale label.

2. The buffer seals should be place on the outside diameter of the tube at a distance

approximately 2cm from the end of the tube. They should be orientated as shown in the

diagram below.

Figure 1.6 Diagram showing the location of the fixed bed support

3. Locate the tube in the bottom column flange. Ensure it is located centrally and evenly

4. Replace the top column flange and locate the tube support bolts.

5. Locate the upper and lower buffer seals into the bottom and underside of the top

column flange.

6. Using the correct Allen key tighten the Tube support Rod bolts located in the top

column flange.

Fixed Assembly

1. Install to antijet in the flowcell by carefully locating the 3 pins into the ribbed face of the

flowcell.

2. Locate the flowcell on the cell backing plate and replace the fixing bolts and tighten.

3. Install the process flowtube into the back of the flowcell. Ensure the flowtube seal is

located. Tighten process flowtube using correct tools.

4. Refit the pipespool clamp and pipespool elbow.

5. Install the bed support/seal assembly on to the flowcell by carefully locating the bed

support seal onto the flowcell. The bed support should be fitted with the smooth side

facing into the column. See Figure below.

Figure 1.7 Diagram showing the location of the fixed bed support

Top Buffer

Seal

Bottom Buffer

Seal

Rough Side

Smooth Side

Flow cell