BD LSR II Benutzerhandbuch - Seite 7

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Clean-up

1. Run BLEACH on HIGH for 5 minutes

2. Run WATER on HIGH for 5 minutes

3. Leave the machine in STANDBY and LOW with a tube of WATER on the SIP.

IF you ran BSL-2 samples follow the additional cleaning steps below:

4. Spray cavicide on all surfaces. Contact time for cavicide is 5 minutes unless your APB requires a

longer period. Please let staff know if different contact times are needed.

5. Fill-out the biosafety clipboard next to the machine. List the biohazard, user, lab and clean up

procedure.

6. Empty and flush the tupperware catch basin under the sample tube with bleach, then water, and dry

with paper towels; dispose of the paper towels in the biohazard waste container.

SAVING YOUR DATA (this can be done during Cleanup, to save time)

1. Click on the name of your experiment.

2. Select ExportàExperiment

3. Make sure your data is being exported to C:\Export

4. Click OK

5. On the Desktop, click on FACS Data CHECKIN

6. The appropriate experiment should be displayed, but if not select it.

7. Click OK

8. Find your name and Click OK

Check the Schedule—Is someone running less than 2 hours after you today?

If Yes, leave the machine as is, in STANDBY.

If No, follow the shutdown instructions.

Shutdown

(Only do this if you are the last person of the day!)

1. Make sure you have followed the cleaning instructions

2. Turn off the flow cytometer.

3. Clean up your work area.

Basic Troubleshooting

Always tell the FACS Facility if there are ever any problems with the analyzer, even if these steps resolve it.

If the Cytometer and Computer are not connecting

1. Turn off the computer and cytometer.

2. Turn on the cytometer and wait for 5 minutes then turn on the computer.

The RUN button stays Yellow

1. Check that the FACSFlow Supply System is on.

2. Make sure the sheath is full. Make sure the waste tank is empty.

3. Check to see if your tube is cracked.

I don't see any events!

1. Check your settings.

a. Turn off all gates.

b. Set your Threshold on FCS at no more than 5000.

c. Could also be a software burp. May have to log out of DiVa and log back in

d. Make sure you're in the proper worksheet