BD LSR II Benutzerhandbuch - Seite 8

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If you still see no events the LSRII may be clogged

1. Remove any tubes from the sample probe.

2. Press PRIME. Wait for STANDBY to turn orange.

3. Press PRIME again. Wait for STANDBY to turn orange.

4. Try rerunning a (freshly filtered) sample. If that still doesn't work:

5. Put a NEW tube of BLEACH one the instrument and RUN on HIGH for 5'. Mark the level on the tube and

watch the volume - it should decrease.

6. If the volume level decreases, put a NEW tube of WATER on the instrument and RUN on HIGH for 5'.

7. Try the 197 beads again using the bead settings

8. If that still does not work, ask for help!

Rules

Data Rules

1. Do NOT use USB sticks, thumb drives, external hard drivers or any other form of USB with the

computer.

2. Do NOT connect to the internet from this computer.

3. You MUST export your experiment.

Running the Machine Rules

4. You must FILTER your cells before running.

5. You MUST follow the cleaning procedure.

6. Leave the area neat and tidy.

Notes on Data

• Check in your data to protect it

• Data is deleted monthly. If it is not checked in it is gone.

• USB and personal back up devices are not allowed on our machines.

• Discs may be burned when alternatives are needed.

• A .jo file for MAC (legacy Flowjo 9.75 and older) and a .wsp (version 10 both Mac and PC). Will be sent

to you through the data archive once your data is checked in

• Currently we are recommending FlowJo version 10.2

• Start FlowJo and from the FileMenu select Open and then open your .wsp file.

• You will be prompted for Username: 'flowjo' Password: '314159'.