Olympus BX60 Anleitung Handbuch - Seite 34

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BX60

Use-of Immersion Objectives

(Fig. 68)

1. Focus on the specimen with a low power objective.

2. Place a drcpol immersion oil (provided] onto the specimen at the area

to be observed.

3. Turn the revolving nosepiece to engage the immersion objective, then

focus using the fine adjustment knob.

* Since any bubbles In the oil will impair the image, make sure

that the oil is free of bubbles,

a. To check for bubbles, remove the eyepiece and fuffy open the field

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inside the observation tube. (The pupil should appear round and bright)

b. To remove bubbles, rock the nosepecesJghtiy to move the al immersion

objective beck and forth a few times.

O If the condsnser marking shows a numerical aperture INA) of 1.0 or

more, the number applies only when oil is present between the slide

glass and the top surface of the condenser. When oil is not present

the NA is about 0.9.

4. After use. remove oil from the objective front lens by wiping with

gauze sfightty moistened with an ether (7 partsVslcohol (3 pans) mixture

or with xytene.

* Using too much xylene can dissolve the lens adhesive.

O Use a Binocular observation tube (U-TR30. U-SWTRI for photomicrography.

Piwtomicroflnjphy can be performed using the PM-10. the f>4-20.ortfiePM«photomieroo^phicsvstBm.ftc«idures

for operating the photomicrographic units are described in their respective instruction manuals. Procedures specific

to this microscope are described below.

H Attaching Straight/ Photo Tube (U-SPT) - (Fig. 59)

1. Using the Allen screwdriver, loosen the damping screw (T) on the

trinocular tube photo port.

photo tube, then mount the straight photo tube on the trinocular tube

photo port.