Nikon eclipse e100 Anleitung Handbuch - Seite 24

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Miscellaneous Operations

The "Oil" mark on the side of an objective indicates that it is an oil-immersion type objective. (The

oil-immersion objective also has a black band around the barrel end.) An oil-immersion objective

is used with the immersion oil applied between the front of the objective and the coverglass. For

an oil-immersion objective with a numerical aperture of 1.0 or more, use of an oil-immersion type

condenser is required to take full advantage of its performance. An oil-immersion type condenser,

like an oil-immersion type objective, needs immersion oil to be applied between the front of the

condenser and the coverglass.

The abbe condenser included in the bright viewfield set can be used for oil immersion observation.

Example of Oil-Immersion

Condenser:

Move the specimen toward the back and

lower the condenser slightly. Add a drop

of oil on the front of the condenser from

the long hole on the stage. Bring the

specimen back over the condenser and

slowly raise the condenser.

Objective:

Rotate the revolving nosepiece to move

the objective out of position. Add a drop

of oil to the specimen. Slowly rotate the

revolving nosepiece to bring the objective

back into position.

Eliminate Air Bubbles

Make sure that air bubbles are not trapped during oil application. Air bubbles degrade the

image. To see if any air bubbles are trapped in the oil, remove one eyepiece and fully open the

aperture diaphragm. Look into the eyepiece tube and check the objective pupil (a bright round

part). If you cannot see it well, replace one of the eyepieces with the centering telescope

(optional) and look through the eyepieces of the centering telescope while rotating the eyepiece

part of the centering telescope.

Do any of the following to eliminate air bubbles:

• Rotate the revolving nosepiece to move the objective back and forth.

• Gently move the condenser vertical motion lever to move the condenser up and down.

• Add another drop of oil.

• Wipe off the oil and apply again.