BD FACSAria II Manual de consulta rápida - Página 3
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Imprimir páginaSorting Setup
Do the following to set up for sorting. For more details, refer to Chapter 5
in the user's guide.
1
Check the laser delay and area scaling factors for your sheath pressure.
Refer to Checking Performance with Cytometer Setup and Tracking in
Chapter 4 in the user's guide or perform quality control as described in
Appendix E.
2
Optimize cytometer settings for the sample to be sorted.
Refer to Data Collection in Chapter 4 in the user's guide.
3
Install the required collection device and set up the side streams.
4
Use BD FACS™ Accudrop to calculate the drop delay.
5
Run the sample to be sorted. Use gating tools and subsetting methods
to define the population(s) of interest.
NOTICE
Snap-to gates cannot be used for sort gates.
6
Define a sort layout for the tube containing the defined sort
populations.
Sorting
1
Install the collection tubes, plate, or slide.
2
Install the sample tube onto the loading port and click Load.
3
Verify that the current tube pointer is set to the appropriate tube in the
Browser and click Sort.
4
Click OK if you are prompted to open the aspirator drawer or turn on
the deflection plates.
3