Olympus bx40 Manual de instrucciones - Página 22

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Olympus bx40 Manual de instrucciones
Aperture
iris
diaphragm
Fig. 35
Fig. 36
Apetture
Iris Diaphragm
(Figs. 35.36)
1
l
The
aperture
iris diaphragm determines the numerical aperture of the
illumination System. Matthing the numerical aperture of the illumination
System with that of the objective provides better image resolution and
contrast, and also increases the depth of focus.
l
Since the contrast of microscope specimens is ordinarily low, setting
the condenser aperture iris diaphragm to 7060%
of the N.A. of the
objective in use is usually recommended.
If necessary, adjust the ratio
by removing the eyepiece and looking into the eyepiece sleeve while
adjusting the aperture iris diaphragm ring @ until the image shown in
Fig. 35 is Seen.
1 Usina the Numerital
Aoet-ture Scale
Set the condenser numerical aperture scale @ to about 80% of the NA
value @ of the respective objective. (Fig. 36)
Example:
With the Plan 40X (NA 0.651, set the scale to 0.65 x 0.8 = 0.5.
Compatibility
of Objectives
and Condensers
IO-60X
1 oox
Applicable
Applicable
Top lens in light path
Condenser NAslightly
lessthanperfect**
*l When using the U-SC Swing-out condenser together with a 2X or 4X objective, fully
open the condenser aperture iris diaphragm and use the field iris diaphragm in the base
as aperture diaphram.
** The slightly lower NA results in a somewhat darker field of view with a IOOX objective,
but the combination is acceptable.
l
To obtain better illumination, use of the U-ULC is recommended
in photomicrography
when using the 2X or 4X objective.
17
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