Olympus bx40 Manuel d'instructions - Page 22

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Aperture

iris

diaphragm

Fig. 35

Fig. 36

Apetture

Iris Diaphragm

(Figs. 35.36)

The

aperture

iris diaphragm determines the numerical aperture of the

illumination System. Matthing the numerical aperture of the illumination

System with that of the objective provides better image resolution and

contrast, and also increases the depth of focus.

Since the contrast of microscope specimens is ordinarily low, setting

the condenser aperture iris diaphragm to 7060%

of the N.A. of the

objective in use is usually recommended.

If necessary, adjust the ratio

by removing the eyepiece and looking into the eyepiece sleeve while

adjusting the aperture iris diaphragm ring @ until the image shown in

Fig. 35 is Seen.

1 Usina the Numerital

Aoet-ture Scale

Set the condenser numerical aperture scale @ to about 80% of the NA

value @ of the respective objective. (Fig. 36)

Example:

With the Plan 40X (NA 0.651, set the scale to 0.65 x 0.8 = 0.5.

Compatibility

of Objectives

and Condensers

IO-60X

1 oox

Applicable

Top lens in light path

Condenser NAslightly

lessthanperfect**

*l When using the U-SC Swing-out condenser together with a 2X or 4X objective, fully

open the condenser aperture iris diaphragm and use the field iris diaphragm in the base

as aperture diaphram.

** The slightly lower NA results in a somewhat darker field of view with a IOOX objective,

but the combination is acceptable.

To obtain better illumination, use of the U-ULC is recommended

in photomicrography

when using the 2X or 4X objective.