Olympus Fluoview FV1000 Visión general - Página 10

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New Objective with Low Chromatic Aberration Delivers

World-Leading Imaging Performance.

Low Chromatic Aberration Objective

NEW

Best Reliability for Colocalization Analysis

A new high NA oil-immersion objective minimizes chromatic

aberration in the 405–650 nm region for enhanced imaging

performance and image resolution at 405 nm. Delivers a high

degree of correction for both lateral and axial chromatic

aberration, for acquisition of 2D and 3D images with excellent

and reliable accuracy, and improved colocalization analysis. The

objective also compensates for chromatic aberration in the near

infrared up to 850 nm.

Lateral and Axial Chromatic Aberration

Small Degree of

Chromatic Aberration

Performance Comparison of PLAPON 60xOSC and UPLSAPO 60xO

Axial chromatic

aberration (Z direction)

Compared for PSF fluorescent

beads (405 nm, 633 nm).

Lateral chromatic

aberration (X-Y direction)

Compared for PSF fluorescent

beads (405 nm, 488 nm, 633

nm).

3D image

Tubulin in Ptk2 cells labeled with

two colors (405 nm, 635 nm) and

compared.

Large Degree of

Chromatic Aberration

Axial chromatic

aberration

Lateral chromatic

(Z direction).

aberration (X-Y

direction at FN6).

PLAPON60xOSC

UPLSAPO60xO

Approx.

0 µm

Approx. 0.1 µm

Approx. 0.2 µm

Low Chromatic

Aberration Objective Magnification: 60x

Objective

Chromatic Aberration Comparison for PLAPON 60xOSC and

UPLSAPO 60xO

0.5

0.0

-0.5

400

*Chromatic aberration values are design values and are not guaranteed values.

Approx.

0.5 µm

Improved Flatness and Resolution at 405 nm

Better flatness reduces the number of images for tiling.

PLAPON60xOSC

NA: 1.4 (oil immersion)

W.D.: 0.12 mm

Chromatic aberration compensation range: 405–650 nm

Optical data provided for each objective.

Lateral Chromatic Aberration

UPLSAPO60xO

PLAPON 60xOSC

450

500

550

Wavelength (nm)

Flatness Comparison Image at 1x Zoom

UPLSAPO60xO

650

600