Olympus Fluoview FV1000 Visión general - Página 8
Navegue en línea o descargue pdf Visión general para Microscopio Olympus Fluoview FV1000. Olympus Fluoview FV1000 28 páginas. Confocal microscope
También para Olympus Fluoview FV1000: Manual del usuario (42 páginas), Instrucciones breves (7 páginas), Instrucciones breves (7 páginas), Manual del usuario (9 páginas), Manuallines (3 páginas)
Imprimir página
Two Versions of Light Detection System that Set New Standards
for Optical Performance.
Spectral Based Detection
Flexibility and High Sensitivity
Spectral detection using gratings
for 2 nm wavelength resolution
and image acquisition matched to
fluorescence wavelength peaks.
User adjustable bandwidth of
emission spectrum for acquiring
bright images with minimal cross-
talk.
Precise Spectral Imaging
The spectral detection unit uses a grating method that offers
linear dispersion compared with prism dispersion. The unit
provides 2 nm wavelength resolution to high-sensitivity
photomultiplier tube detectors. Fluorescence separation can be
achieved through unmixing, even when cross-talk is generated
by multiple fluorescent dyes with similar peaks.
Filter Based Detection
Enhanced Sensitivity
Three-channel scan unit with detection system featuring hard
coated filter base. High-transmittance and high S/N ratio optical
performance is achieved through integration of a pupil projection
lens within the optics, the use of a high sensitivity photomultiplier
and an analog processing circuit with minimal noise.
High-Performance Filters Deliver Outstanding Separation
Special coatings deliver exceptionally sharp transitions to a
degree never achieved before, for acquisition of brighter
fluorescence images.
DM488/543/633 Comparison
100
80
60
40
20
0
480
500
520
540
560
Wavelength (nm)
Conventional mirror unit
580
600
620
640
660
680
High-performance mirror unit
EGFP–EYFP Fluorescence Separation
2,600
2,400
2,200
2,000
1,800
EYFP
1,600
1,400
1,200
1,000
800
600
400
496
500
504
508
512
516
520
Wavelength
700
7
EGFP
EGFP (dendrite) — EYFP (synapse)
XY
Wavelength detection range: 495 nm–561 nm in
524
528
532
536
540
544
548
552
2 nm steps
Excitation wavelength: 488 nm
Courtesy of: Dr. Shigeo Okabe
Department of Anatomy and Cell Biology,
Tokyo Medical and Dental University