Olympus Fluoview FV1000 Panoramica - Pagina 8
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Two Versions of Light Detection System that Set New Standards
for Optical Performance.
Spectral Based Detection
Flexibility and High Sensitivity
Spectral detection using gratings
for 2 nm wavelength resolution
and image acquisition matched to
fluorescence wavelength peaks.
User adjustable bandwidth of
emission spectrum for acquiring
bright images with minimal cross-
talk.
Precise Spectral Imaging
The spectral detection unit uses a grating method that offers
linear dispersion compared with prism dispersion. The unit
provides 2 nm wavelength resolution to high-sensitivity
photomultiplier tube detectors. Fluorescence separation can be
achieved through unmixing, even when cross-talk is generated
by multiple fluorescent dyes with similar peaks.
Filter Based Detection
Enhanced Sensitivity
Three-channel scan unit with detection system featuring hard
coated filter base. High-transmittance and high S/N ratio optical
performance is achieved through integration of a pupil projection
lens within the optics, the use of a high sensitivity photomultiplier
and an analog processing circuit with minimal noise.
High-Performance Filters Deliver Outstanding Separation
Special coatings deliver exceptionally sharp transitions to a
degree never achieved before, for acquisition of brighter
fluorescence images.
DM488/543/633 Comparison
100
80
60
40
20
0
480
500
520
540
560
Wavelength (nm)
Conventional mirror unit
580
600
620
640
660
680
High-performance mirror unit
EGFP–EYFP Fluorescence Separation
2,600
2,400
2,200
2,000
1,800
EYFP
1,600
1,400
1,200
1,000
800
600
400
496
500
504
508
512
516
520
Wavelength
700
7
EGFP
EGFP (dendrite) — EYFP (synapse)
XY
Wavelength detection range: 495 nm–561 nm in
524
528
532
536
540
544
548
552
2 nm steps
Excitation wavelength: 488 nm
Courtesy of: Dr. Shigeo Okabe
Department of Anatomy and Cell Biology,
Tokyo Medical and Dental University