Olympus U-UCD8 Manual de instrucciones - Página 20

Navegue en línea o descargue pdf Manual de instrucciones para Equipos de laboratorio Olympus U-UCD8. Olympus U-UCD8 40 páginas. Universal condenser

4. Rotate the polarizer rotation knob to achieve a perfectly black field and then tighten the clamping knob.

}The "crossed Nicol" (perfectly dark) position is located near the 0

5. Place the specimen on the stage and move the stage up and down to bring the specimen into focus.

6. Adjust the field iris diaphragm opening until it circumscribes the field of view.

7. Stopping down the aperture iris diaphragm may further increase the contrast of the image.

1 7

Fig. 4

General Precautions in Operation

1. If reflected light fluorescence microscopy in the U-excitation

mode is performed using a 10X or 20X objective with the

condenser engaged, flare may become prominent depend-

ing on the specimen condition.

If this occurs and if you do not need transmitted light DIC, either

lower the condenser or use the light shield sheet @ provided

with the reflected light fluorescence attachment. (Fig. 4)

2. When the polarizer rotation knob is set to the 0

dex, the polarizer and analyzer are nearly at the "crossed Nicol"

position. However, since a perfect "crossed Nicol" position can

hardly be achieved due to slight unavoidable positioning er-

ror of the intermediate tube, always perform fine adjustment.

3. When the top lens is swung out, the field of view become

obscured if the aperture iris diaphragm is stopped down (when

using a 2X or 4X objective).

4. Due to the large numerical aperture (N.A.), the U-DIC60, U-

DPO60S, U-DIC100 or U-DP100 DIC prism for 60X and 100X

objectives can also be used for brightfield observation.

position index.

position in-