Nikon Live Cell 2 Manual - Página 6

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Adjusting Kӧhler illumination (Transmitted light)

For transmitted light imaging it is essential to optimise the condenser alignment to ensure that the full numerical

aperture of the microscope system can be realised (to provide an even illumination of the sample). It is therefore

important to ensure that the light passes through the condenser optimally to allow for high quality imaging.

N.B. Kӧhler Illumination adjustment should only be conducted through the eyepiece.

Field stop iris

diaphragm

Condenser

focus

Condenser

lens

1. Through the eyepiece, focus on your. Fully close the field iris by turning it anticlockwise. (Fig. 14)

2. Using the adjustment knob, move the condenser whilst looking through the eyepiece until you see the

octagonal field iris. Turning the knob towards you to bring the condenser lens close to the sample. Adjust the

condenser focus so that the outline of the field iris is sharp and in focus. (Fig. 15)

3. If required, centre the field iris in the eyepiece using the adjustment screws on the condenser unit.

4. Once centred, open the field iris again so that the field iris edges are just outside of the field of view.

Alignment screws (use

allen key if centering

required)

Nikon Imaging Centre @ King's College London

Nikon

Imaging Centre

Fig. 13

King's College London