Olympus ST. HSC Manuel d'instructions - Page 8

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2. Focusing
Afte~
completion of the centering of the illuminating system, place the specimen on the stage and focus the
microscope on the specimen. If you move the body tube up and down while looking through the eyepiece. you
may jam the objective down on to the specimen, and you could quite easily crack the specimen and the front
lens as well. First, use a low· power objective, and operate the coarse adjustment knob to lower the body tube
until the
tip
of the objective comes close to the specimen. All this must be done while looking at the microscope
from the side.
Next, look into the eyepiece and raise the body tube to roughly focus the microscope on the
specimen, and fasten the stopper at this point.
ext, turn the nosepiece to chilnge to an objective with a desired
magnification. Usc the fine adjustment knob to get the specimen sharply in focus_ Move the specimen by hand
or operate the mechanical stage to bring the target area of the specimen into the center of the field of view.
3. Change of magnification
The magnifying power of the microscope is changed either by changing the eyepiece or by changing the
objective.
I) Change of the eyepiece
Eyepieces with different magnifications arc inserted into the eyepiece sleeve to change the magnifying power
of the microscope. In this case, the microscope remains roughly in focus but further focusing is required to
obtain a sharply focused image.
2) Change of the Objective
When the objective is changed to change the magnification of the microscope, you first focus the microscope
with
a
low-power Objective and then turn the nosepiece to change to a higher-power objective. In Model HS
you need only operate the fine adjustment knob to obtain an accurate focus.
4. Resolving Power
The resolving power of
a
microscope depends solely on that of the objective and has nothing to do with the
eyepiece. The only function of the eyepiece is to magnify the image resolved by the objective. This means that
no matter how much the final magnification may be increased by using a high-power eyepiece, you will never
see what is not shown in the image that is formed by the objective. For example, you get the same magnifiC'dtion
of 800X by using a 40X objective and a 20X eyepiece, and also by using a IOOX objective and a 8X eyepiecc.
However. the latter combination has a resolving power twice higher and show more details than the former
combination.
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