Olympus ST. HSC Petunjuk Manual - Halaman 8

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2. Focusing

Afte~

completion of the centering of the illuminating system, place the specimen on the stage and focus the

microscope on the specimen. If you move the body tube up and down while looking through the eyepiece. you

may jam the objective down on to the specimen, and you could quite easily crack the specimen and the front

lens as well. First, use a low· power objective, and operate the coarse adjustment knob to lower the body tube

until the

tip

of the objective comes close to the specimen. All this must be done while looking at the microscope

from the side.

Next, look into the eyepiece and raise the body tube to roughly focus the microscope on the

specimen, and fasten the stopper at this point.

ext, turn the nosepiece to chilnge to an objective with a desired

magnification. Usc the fine adjustment knob to get the specimen sharply in focus_ Move the specimen by hand

or operate the mechanical stage to bring the target area of the specimen into the center of the field of view.

3. Change of magnification

The magnifying power of the microscope is changed either by changing the eyepiece or by changing the

objective.

I) Change of the eyepiece

Eyepieces with different magnifications arc inserted into the eyepiece sleeve to change the magnifying power

of the microscope. In this case, the microscope remains roughly in focus but further focusing is required to

obtain a sharply focused image.

2) Change of the Objective

When the objective is changed to change the magnification of the microscope, you first focus the microscope

with

low-power Objective and then turn the nosepiece to change to a higher-power objective. In Model HS

you need only operate the fine adjustment knob to obtain an accurate focus.

4. Resolving Power

The resolving power of

microscope depends solely on that of the objective and has nothing to do with the

eyepiece. The only function of the eyepiece is to magnify the image resolved by the objective. This means that

no matter how much the final magnification may be increased by using a high-power eyepiece, you will never

see what is not shown in the image that is formed by the objective. For example, you get the same magnifiC'dtion

of 800X by using a 40X objective and a 20X eyepiece, and also by using a IOOX objective and a 8X eyepiecc.

However. the latter combination has a resolving power twice higher and show more details than the former

combination.

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