Olympus SZX16 Manuel d'instructions - Page 20

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Using the Exciter Balancer U-EXBABG/EXBAUB/EXBAUG

}When observing fluorescence generated with U/B/G multiple excitation using double-dye fluorescence excitation and a

barrier filter, the exciter balancer can be used to adjust the balance between the intensities of the excitation light of the

fluorescent dyes.

Engagement adjustment lever

Pushed in: IN.

Pulled out: OUT.

Rotation: Adjustment (45°)

Fig. 18

Clamping screw

Attaching Procedure

1. Remove the dummy from the exciter balancer insertion slot.

2. Set the balancer's engagement adjustment lever @ in the vertical position

and insert the balancer into the insertion slot ² on the right side of the

illuminator.

3. Tighten the clamping screw ³ using the Allen screwdriver to fix the balancer.

Operating Procedure

1. Mount a commercially available double-dye fluorescence excitation filter

and a barrier filter in the slider position (see page 16 for the attaching

method).

2. Rotate the turret to engage these filters in the light path.

# Due to the characteristics of G-excitation, its brightness adjustment

range is lower than that of the B- or U-excitation. The brightness

adjustment range is also variable depending on the variance in

characteristics of the filter.

# Due to the filter rotation angle and the variance in characteristics of

the filter, irregularities in illumination may be observed on the top

and bottom of the visual field. But these irregularities do not affect

the area captured in photomicrography.