Nikon eclipse e100 Manuel d'instructions - Page 18

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Nikon eclipse e100 Manuel d'instructions
Turning the focus knobs recklessly is a long and hard way to focus on the image. If you are
using a high power objective, you may even damage the specimen by pressing it against the
objective. Before breaking the coverglasses or damaging the objectives, read the following
and find the correct way to focus on the specimen.
(1) Put the 10x (or 4x) objective in the optical path.
(2) Turn the coarse focus knob to raise the stage to its upper limit. *
(3) Looking into the eyepieces, slowly rotate the coarse focus knob to lower the
stage. When the specimen image appears, stop rotating the knob.
(4) Rotate the fine focus knob and precisely focus on the image.
When you want to observe the image with a high power objective, first focus on
the image using a 10x (or 4x) objective. Then change to a high power objective
and rotate the fine focus knob for precise focusing.
1. When rotating the coarse focus knob while looking into the eyepieces, be sure to
turn it only in the direction that lowers the stage.
2. When raising the stage using the coarse focus knob, take your eyes off the
eyepieces and look at the gap between the upper surface of the specimen and the
front of the objective from the side.
3. First focus with a low power objective. Then change to a high power objective.
*1: Since the working distances of 10x and 4x objectives are large (p. 33), these objectives do not touch
the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses
of a standard thickness are used. (The standard thickness for slides is 1.2 mm and that for coverglass
is 0.17 mm.)
16
Focusing
First raise the stage to the most extent
not to hit against the objective,
1
then look into the eyepieces
and lower the stage.