Nikon eclipse e100 Petunjuk Manual - Halaman 18

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Turning the focus knobs recklessly is a long and hard way to focus on the image. If you are

using a high power objective, you may even damage the specimen by pressing it against the

objective. Before breaking the coverglasses or damaging the objectives, read the following

and find the correct way to focus on the specimen.

(1) Put the 10x (or 4x) objective in the optical path.

(2) Turn the coarse focus knob to raise the stage to its upper limit. *

(3) Looking into the eyepieces, slowly rotate the coarse focus knob to lower the

stage. When the specimen image appears, stop rotating the knob.

(4) Rotate the fine focus knob and precisely focus on the image.

When you want to observe the image with a high power objective, first focus on

the image using a 10x (or 4x) objective. Then change to a high power objective

and rotate the fine focus knob for precise focusing.

1. When rotating the coarse focus knob while looking into the eyepieces, be sure to

turn it only in the direction that lowers the stage.

2. When raising the stage using the coarse focus knob, take your eyes off the

eyepieces and look at the gap between the upper surface of the specimen and the

front of the objective from the side.

3. First focus with a low power objective. Then change to a high power objective.

*1: Since the working distances of 10x and 4x objectives are large (p. 33), these objectives do not touch

the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses

of a standard thickness are used. (The standard thickness for slides is 1.2 mm and that for coverglass

is 0.17 mm.)

Focusing

First raise the stage to the most extent

not to hit against the objective,

then look into the eyepieces

and lower the stage.