Nikon N-STORM Manuel d'utilisation - Page 11

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Place the imaging target in the visual field.

Set a specimen, direct the light path to the binocular part to

perform epi-fluorescence microscopy, and then put the

STORM imaging target into the visual field.

(For details on the procedure for epi-fluorescence

microscopy, see the user manual for the microscope.)

Configure camera settings.

In the [DU-897 Settings] control window (EM-CCD camera

settings), configure the settings as follows.

[Format For Live]:

[Format For Capture]: No Binning

Exposure time:

[Readout Mode]:

[EM Gain Multiplier]:

[Conversion Gain]:

[Desired Temperature]

(Commands -> Advanced

Camera Settings):

Wait until the temperature of the camera stabilizes at

about -70°C and [Desired temp. differs!] disappears.

It takes a few minutes for the temperature of the camera to

stabilize.

Put the objectives for STORM into the light path.

Objectives:

CFI Apo TIRF 100x oil (NA1.49) or

CFI Plan Apo VC 100x oil (NA1.40)

Use Nikon immersion oil Type B or Type NF for oil

immersion of the objectives.

When using CFI Apo TIRF 100x oil, adjust the correction

ring to suit the cover glass. If a cover glass No. 1-S

(No.1.5) is used, it is recommended that the position of the

correction ring be 0.165 mm.

Chapter 2

STORM Microscopy Operation

No Binning

Any setting (50 msec or 1

frame recommended)

EM Gain 10 MHz 14-bit

Maximum available value

-70°C