Olympus FV3000 Panduan Keselamatan

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Halaman Cetak

BEGINNERS SAFETY MANUAL FOR

OLYMPUS FV3000 CONFOCAL (#02-20)

Produced by Shaalini, Graham Wright, Fiona Chia, Cris Barzaghi, Melvin

1. Safety Guidelines ................................................................................................................... 2

1.1 Lasers ............................................................................................................................... 2

1.2 Immersion Oil ................................................................................................................... 2

1.3 Metal Halide Lamps (MHL) .............................................................................................. 2

2. Training .................................................................................................................................. 3

2.1 To arrange a training session: .......................................................................................... 3

3. Online Booking and System Access........................................................................................ 4

3.1 Acknowledgements .......................................................................................................... 5

4. Operation Procedures ............................................................................................................ 5

4.1 Switching ON Protocol ..................................................................................................... 5

4.2 Objective lens ................................................................................................................... 6

4.2 Software Initiation ........................................................................................................... 7

4.3 Setting up directory to save images ................................................................................ 8

4.4 Laser Configuration Settings ............................................................................................ 9

4.5 Using the LSM Imaging Tab ............................................................................................ 10

4.6 Image Adjustments - Setting the Gain and Offset properly.......................................... 11

4.7 How to do a Z stack for a 3D Image (xyz) ....................................................................... 11

4.8 For Time-lapse (xyzt) ...................................................................................................... 12

4.9 Reuse previous settings and export images .................................................................. 12

4.10 Switching OFF Protocol ................................................................................................ 13

5. Troubleshooting ................................................................................................................... 14

Last updated on Mar 2022